Durable Expression of Minicircle DNA-Liposome-Delivered Androgen Receptor cDNA in Mice with Hepatocellular Carcinoma
Figure 2
In vitro expression and functional analysis of AR minicircle DNA transfection. (a) AR protein expression in the minicircle DNA transfected 293T cells by immunoblot. The left lane is MC.GFP transfectant where the right lane is MC.hAR transfectant. Actin served as the loading control. (b) AR transactivation function was measured by ARE-luciferase assay. The MC.GFP and MC.hAR DNAs were cotransfected with ARE-luciferase into 293T cells and treated with either vehicle (ethanol; EtOH) or dihydrotestosterone (DHT, 10 nM) for 24 hrs. Cell lysate were harvested to analyze the dual-luciferase assay as described in Section 2. The readings of each group were normalized with those of the MC.GFP-EtOH group and plotted on a graph. The data came from at least 3 independent experiments.
