Durable Expression of Minicircle DNA-Liposome-Delivered Androgen Receptor cDNA in Mice with Hepatocellular Carcinoma
Figure 3
Long-term in vitro expression of minicircle DNA in 293T cells. MC.hAR or MC.GFP was transfected into 80% confluence 293T cells, and green fluorescence was observed under fluorescence microscopy at days 2, 6, 10, and 14. The upper panels show MC.GFP transfectant and the lower panels show MC.hAR transfectant. The cells were 1/10 subcultured every three days upon cell reach to around 80~90% confluence. The strong GFP signals were still detectable on day 14 (fourth passage of subcultures).
