Comparison of Extraction Methods of Chitin from Ganoderma lucidum Mushroom Obtained in Submerged Culture

Table 1

Conditions for the chitin isolation.

Assay	Treatment 1	Treatment 2	Treatment 3
A1	Dried and powdered biomass	Deproteinization; NaOH; 1 M; 1 : 30 (w/v); 40°C, 2 h
A2	Dried and powdered biomass	Deproteinization; NaOH; 1 M, 2 M; 1 : 30 (w/v); 90°C, 2 h
A3	Dried and powdered biomass	Deproteinization; NaOH; 2 M, 4 M; 1 : 30 (w/v); 90°C, 2 h	Decolorization KMnO4; 10 g L^−1, 1 h; C2H2O4; 10 g L^−1, 1 h
A4	Dried and powdered biomass, immersion in hot water in order to remove polysaccharides	Deproteinization; NaOH; 2 M, 4 M, 6 M, and 8 M; 1 : 20 (w/v); 100°C, 3 h	Decolorization KMnO4; 10 g L^−1, 1 h; C2H2O4; 10 g L^−1, 1 h
A5	Dried and powdered biomass and sonication 40 min washed with ethanol 24 h	Deproteinization; NaOH; 4 M; 1 : 20 (w/v); 100°C, 3 h; Three times.	Decolorization; KMnO4; 10 g L^−1, 1 h; C2H2O4; 10 g L^−1, 1 h