Crystal Structure of a Conserved Hypothetical Protein MJ0927 from Methanocaldococcus jannaschii Reveals a Novel Quaternary Assembly in the Nif3 Family
Figure 4
(a) EMSA analysis of MJ0927. 25 nM ssDNA was incubated with various amounts of MJ0927 at 0, 50, 250, and 500 μM in a 10-μL reaction mixture and 25 nM dsDNA was incubated with increasing amounts of MJ0927 at 0, 50, 100, and 200 μM. (b) Surface representations displaying the electrostatic potential of MJ0927. Electrostatic potential was calculated using the program APBS [19] implemented in PYMOL (The PyMOL Molecular Graphics System, Version 0.99rc6 Schrödinger, LLC.). Positive potential is shown in blue, neutral in white and negative potential in red. Green arrows indicate possible paths for DNA entry.
