Rapid and Sensitive PCR-Dipstick DNA Chromatography for Multiplex Analysis of the Oral Microbiota

Table 2

Procedure for semiquantification of plaque samples.

Procedure	Item	Amount (volume)
Plaque sampling	Dental plaque	1.5 mg
DNA extraction	InstaGene Matrix Kit	300 µL
PCR	DNA extraction used for PCR	21 µL
	Primer F (tagged) (5 µM)	2 µL
	Primer R (biotinylated) (5 µM)	2 µL
	Hot StarTaq PLUS Master Mix	25 µL
Dipstick DNA chromatography	DNA amplified by PCR	1 µL
	Deionized water	29 µL
	Eluent (supplied by TBA Co.; containing detergents, blocking agents, PBS, and salts solution)	10 µL
	Streptavidin-coated blue latex suspension (supplied by TBA Co.)	2 µL