Effect of Cyclic Mechanical Stimulation on the Expression of Osteogenesis Genes in Human Intraoral Mesenchymal Stromal and Progenitor Cells

Table 2

Expression of the osteogenesis-specific markers affected by mechanical stimulation for 7 and 14 days.

	Day 7				Day 14
	EX(−)	EX(+)	OG(−)	OG(+)	EX(−)	EX(+)	OG(−)	OG(+)
RUNX2	1	1.01±0.3	0.98±0.3	1.40±0.5	1	0.75±0.3	2.48±0.6	3.13±1.1
Col1A1	1	1.16±0.3	1.70±0.8	2.92±1.1; P = 0.0039	1	0.68±0.4	0.94±0.4	2.02±0.9; P = 0.049
SPP1	1	0.95±0.3	1.11±0.4	1.91±0.5; P = 0.040	1	1.44±0.7	1.48±0.2	2.92±0.8; P = 0.038
BGLAP	1	0.85±0.1	0.78±0.4	0.84±0.5	1	0.67±0.2	0.91±0.5	1.08±0.2
BMP2	1	1.53±0.8	2.17±1.3	5.54±3.2; P = 0.036	1	2.06±1.0	3.84±1.8	6.48±3.5; P = 0.013
SPARC	1	0.93±0.2	1.47±0.4; P = 0.035	1.60±0.9	1	0.88±0.3	0.95±0.4	2.13±0.8; P = 0.044
ALPL	1	0.86±0.4	5.35±1.9; P = 0.042	4.05±1.8; P = 0.046	1	0.78±0.5	3.55±1.6; P = 0.023	3.62±1.8; P = 0.042

mRNA levels of RUNX2, Col1A1, SPP1, and BGLAP. BMP2 and ALPL were normalized to the undifferentiated mechanically unstimulated control group EX(−) of each day (ratio = 1). EX(+) represents the undifferentiated mechanically stimulated group, OG(−) the osteogenic differentiated mechanically unstimulated group, and OG(+) the osteogenic differentiated mechanically stimulated group. The values are mean ± SD of independent experiments performed in triplicate (𝑛=7); ^∗𝑃<0.05.