Research Article
Effect of Cyclic Mechanical Stimulation on the Expression of Osteogenesis Genes in Human Intraoral Mesenchymal Stromal and Progenitor Cells
Table 3
Quantitation of ARS staining of calcium deposits and ALP enzyme.
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The ARS staining of calcium deposits and measurements of an OD of 570 nm after cetylpyridinium chloride elution were performed at days 7 and 14. Quantitation of calcium deposits revealed a highly significant increase of an OD of 570 nm values in osteogenic differentiated cells (OG) compared to the corresponding undifferentiated controls (EX). Mechanical stimulation revealed a significant increase in calcium deposits in the OG(−) cells compared to the OG(+) cells. Quantitation of ALP enzyme at day 14 revealed a significant increase in osteogenic differentiated cells (OG) compared to the corresponding undifferentiated controls (EX). The values are mean ± SD of independent experiments performed in triplicate (); . |