Destabilization of Akt Promotes the Death of Myeloma Cell Lines
Figure 1
Prolonged exposure to rapamycin also inhibits mTORC2 pathway. (a) U266 cells or (b) Km3 cells were cultured in the presence of 20 nmmol/L rapamycin for the indicated periods of time. Total cellular proteins were assayed by immunoblotting for Akt p-S473 phosphorylation. Actin expression serves as a loading control. The Akt p-S473/actin ratio was calculated by dividing the total pixel volume of Akt by the total pixel volume of actin. (c) U266 cells or (d) Km3 cells were cultured in the presence of 20 nmmol/L rapamycin for the indicated periods of time. Total cellular proteins were assayed by immunoblotting for Akt p-T450 phosphorylation. Actin expression served as a loading control. The Akt p-T450/actin ratio was calculated by dividing the total pixel volume of Akt by the total pixel volume of actin. The results shown are representative of three independent experiments.
