Purification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells

Table 1

Activities induced by B. mattogrossensis snake venom.

Effect^a	Activity
Phospholipase activity^b (U/mg)	1,864.05
Proteolytic activity^c (U/10 𝜇g)	3.0±0.1
Hemorrhagic halo^d (cm^2)	3.33±0.05
Coagulation activity^e (MCD, 𝜇g)	0.325

aAll experiments were carried out in triplicate. bActivity using NOB stained substrate. cOne enzymatic unit (U) was defined as the quantity of enzyme needed to increase the absorbance by 0.05 UA/440 nm. dValues 3 h after incubation with crude venom of B. mattogrossensis (50 𝜇g). eMCD: the minimum coagulation dose was the dose capable of coagulating 200 𝜇L of citrated plasma in less than a minute.