Hypoxia Enhances the Senescence Effect of Bortezomib—The Proteasome Inhibitor—On Human Skin Fibroblasts

Figure 5

Cytochemical staining for SA-β-galactosidase in fibroblasts. The cells were incubated for 12 h, 24 h, and 48 h in normoxic or hypoxic conditions with 25 nmol/L, 50 nmol/L or without bortezomib. A representative photograph (magnification 200x) is presented.