In Vitro Inhibition of Hepatitis C Virus by Antisense Oligonucleotides in PBMC Compared to Hepatoma Cells

Figure 3

Agarose gel electrophoresis HCV nested PCR products in two different types of cells treated with S-ODN1. HCV infected HepG2 cells and PBMC were treated with SODN1 for 24 and 48 hours. Lane M: 50 bp DNA ladder. For HepG2 cells, lanes C (+, −): untreated HCV infected HepG2 cells cultured for 48 h amplified plus and minus strands, respectively, as a positive control, lanes 24 h (+, −): plus and minus strands PCR products, respectively, for HCV infected HepG2 cells treated with S-ODN1 for 24 h, and lanes 48 h (+, −): plus and minus strands PCR products, respectively, for HCV infected HepG2 cells treated with S-ODN1 for 48 h. For PBMC, lanes C (+, −): plus and minus strands PCR products, respectively, for untreated HCV infected PBMC cultured for 48 h as a positive control, lanes 24 h (+, −): plus strand and minus strands PCR products, respectively, for HCV infected PBMC treated with SODN1 for 24 h, and lanes 48 h (+, −): plus strand and minus strands PCR products, respectively, for HCV infected PBMC treated with S-ODN1 for 48 h.