Reliable Assessment and Quantification of the Fluorescence-Labeled Antisense Oligonucleotides In Vivo

Figure 2

Gel retardation assays to evaluate (a) the stable polyplexes formation and (b) the binding strength of polyplexes in acidic pH. Line1: pGa₄D₄₇, line2: pGu₄D₄₀, line 3: pDMAEMA, and line 4: ODNs. (c) Size and (d) zeta potential of polyplexes (■) pGa₄D₄₇, (●) pGu₄D₄₀, and (▲) pDMAEMA.

(a)

(b)

(c)

(d)