Reliable Assessment and Quantification of the Fluorescence-Labeled Antisense Oligonucleotides In Vivo

Figure 4

Change in emission as collected by Cri MAESTRO 500 FL imaging system. (a) Polyplexes and ODNs just prepared, (b) after 24 hours, and (c) ODNs and pGa₄D₄₇ complexes just prepared at N/P ratio of 12 and 48. (d) Absorbance spectra of Alexa 750, free labeled ODNs, and labeled ODNs after complexation with pGa₄D₄₇ at different N/P ratio.