Validation of Reference Genes for Normalization Gene Expression in Reverse Transcription Quantitative PCR in Human Normal Thyroid and Goiter Tissue

Table 3

Dispersion data of raw Cq values for candidate reference genes in goiter and normal thyroid tissue.

Tissue	Symbol gene	Mean Cq	SD	CV (%)	Minimum	Maximum	MFC
Goiter	ACTB	21.88	1.78	8.1	19.78	25.00	1.26
	B2M	20.83	2.59	9.3	18.10	26.11	1.44
	GAPDH	21.18	1.95	9.2	19.54	25.60	1.31
	HPRT1	30.10	3.10	10.3	25.48	35.24	1.38
	SDHA	31.17	2.21	7.1	29.51	34.56	1.17
	YWHAY	27.48	2.47	9.0	22.71	30.93	1.36
Normal	ACTB	23.56	1.92	8.1	19.99	25.69	1.29
	B2M	24.63	5.08	20.6	18.53	32.65	1.76
	GAPDH	22.84	1.78	7.8	20.54	26.20	1.28
	HPRT1	32.12	3.06	9.5	25.65	35.66	1.39
	SDHA	33.94	3.20	9.4	29.54	39.39	1.33
	YWHAY	26.29	2.35	8.9	22.83	29.25	1.28

SD: standard deviation; CV: coefficient of variation; and MFC: maximum fold change (the ratio of the maximum and minimum values).