IL-6 Impairs Myogenic Differentiation by Downmodulation of p90RSK/eEF2 and mTOR/p70S6K Axes, without Affecting AKT Activity
Figure 5
IL-6 perturbs mTOR and p70S6K activities, without affecting AKT activity. C2C12 myoblasts were cultured in GM until subconfluence and then shifted to DM or DM containing 25 ng/mL of recombinant IL-6. (a, e, and f) After 48 h in the DM, cells were harvested and total protein was extracted; cell lysates (70 μg of total proteins) were subjected to Western blotting analysis with the indicated specific antibodies. Each experiment shown is representative of three independent determinations. (b, c, d, g, h, and i) Densitometric analysis of the relative protein levels (obtained from Western blotting analysis), represented as arbitrary units (A.U.) and equalized for total AKT expression (b and c), α-tubulin expression (d), total p70S6K expression (g and h), or total mTOR expression (i). The experiments show means ± standard deviation of three independent determinations. n.s., not significant; ; (Student’s test).
