Strain-Dependent Induction of Human Enterocyte Apoptosis by Blastocystis Disrupts Epithelial Barrier and ZO-1 Organization in a Caspase 3- and 9-Dependent Manner
Figure 5
Flow cytometry analysis of annexin-V-FITC and propidium iodide staining. Representative dot plots of Caco-2 cells preincubated with culture media as negative control, Blastocystis ST-7, ST-4, and 0.5 μM staurosporine as positive control. 2 × 104 cells were analysed in each sample. Values represent mean ± standard error (error bars; ). Caco-2 cells interacting with ST-7 exhibited significantly higher percentage of annexin-V+ and PI− cells (lower right quadrant) compared to cells coincubated with ST-4 or culture media only ( value < 0.01). Values are means ± standard error (error bars) ().
