Impaired Intestinal Mucosal Barrier upon Ischemia-Reperfusion: “Patching Holes in the Shield with a Simple Surgical Method”

Figure 1

Colony counting method on a Petri plate with Luria-Bertani agar media, under UV light. The plate represents the result of culturing 200 μL aliquots prepared from 0.1 g liver sample homogenized with 1 mL sterile physiological saline solution. Twenty-five (arrow) green fluorescent protein-expressing Escherichia coli colony-forming units (CFU) can be identified, which correspond to a 1250 CFU/g concentration of bacteria in the examined sample.