Impaired Intestinal Mucosal Barrier upon Ischemia-Reperfusion: “Patching Holes in the Shield with a Simple Surgical Method”
Figure 2
Representative hematoxylin-eosin stained histological samples of the jejunum in the three experimental groups. The jejunal section of the sham-operated group (a) represents an unimpaired intestinal villi structure. The ischemia-reperfusion- (IR-) group (b) shows several denudated villi with partial digestion, as shown by the black arrow. The intestinal villi also showed massive epithelial lifting, which extended down the sides of the villi in some cases. Increased cellularity of the lamina propria was also detected. The postconditioned group (c) exhibits a more benign picture, with mild to moderate epithelial lifting. Scale bars: 50 μm. The bar graph (d) shows the Chiu scores of the three segments of the small intestine in the different experimental groups. Sixty minutes of superior mesenteric artery occlusion and six hours of reperfusion caused significant histopathological changes in the three segments of the small intestine compared with the sham-operated group. The use of postconditioning at the onset of the reperfusion significantly ameliorated the histologic injury observed in the jejunum and ileum segments of the IR-group. Results are expressed as means ± SD ( in each group). (††): versus sham-group; (): versus IR-group; (): versus IR-group.
