Impaired Intestinal Mucosal Barrier upon Ischemia-Reperfusion: “Patching Holes in the Shield with a Simple Surgical Method”

Table 3

Semiquantitative analysis of intestinal epithelial tight junction components according to immunostained sections.

Assessed intestinal segment	Experimental group	Claudin-2	Claudin-3	Claudin-4	Zonula occludens-1
Duodenum	Sham	0.06±0.2	5	0.2±0.4	3.73±0.4
	IR	2.27±0.4^††	4.67±0.4^†	1.4±0.5^††	3.47±0.5
	PC	2.06±0.2^††	4.7±0.4^†	1.13±0.3^††	3.4±0.5
Jejunum	Sham	0.2±0.4	4.87±0.3	0.2±0.4	3.53±0.5
	IR	3.47±0.5^††	1.33±0.5^††	2.2±0.4^††	1.33±0.6^††
	PC	2.93±0.6^††,∗	4.6±0.5^∗∗	1.8±0.6^††	3±0.5^†,∗∗
Ileum	Sham	0.13±0.3	4.93±0.2	0.27±0.4	3.73±.04
	IR	3.6±0.5^††	1.2±0.4^††	2.53±0.5^††	1.13±0.3^††
	PC	3.06±0.5^††,∗	4±0.7^††,∗∗	2.2±0.4^††	2.4±0.5^††,∗∗

Results are expressed as means ± SD; 𝑛=15 in each group. Nonparametric Kruskal-Wallis test was used for comparison of all groups. The difference between the groups by Mann-Whitney 𝑈 test: (†): 𝑃<0.05 versus sham-group; (††): 𝑃<0.01 versus sham-group; (∗): 𝑃<0.05 versus IR-group; (∗∗): 𝑃<0.01 versus IR-group.
IR: ischemia-reperfusion; PC: postconditioning.