A Mushroom Extract Piwep from Phellinus igniarius Ameliorates Experimental Autoimmune Encephalomyelitis by Inhibiting Immune Cell Infiltration in the Spinal Cord

Figure 3

Piwep treatment attenuates EAE-induced immune cell infiltration into white matter of spinal cord in mice. PFA fixed sections of the thoracic spinal cord were immunohistochemically stained with antibodies against cell surface molecules such as CD4, CD8, F4/80, and CD20. Immunostaining revealed that T cell, B cell, and microglia/macrophage labeled cells extensively infiltrated the white matter of EAE mice (MOG + vehicle). However, Piwep treatment reduced the infiltration of immune cells into the white matter of the spinal cords in EAE mice (MOG + Piwep). Scale bar represents 100 𝜇m.