Circulating Conventional and Plasmacytoid Dendritic Cell Subsets Display Distinct Kinetics during In Vivo Repeated Allergen Skin Challenges in Atopic Subjects
Figure 5
Conventional DCs are recruited early to the skin upon in vivo allergen challenge. Immunofluorescence staining and data analysis were performed as described in Figure 4. Counterstaining was performed with Hoechst (blue, column 1). CDCs were stained with a monoclonal antibody against BDCA-1 and envisioned with a secondary goat anti-mouse antibody conjugated with AF568 (red, column 2). Column 3 is the result of merging columns 1 and 2. Representative microphotographs (×100) of cDCs are shown 24 h after the first challenge at the diluent (a) and allergen sites (b) and after the sixth challenge at the allergen site (c). Tissue cDCs significantly increased 24 h after the first allergen challenge compared to the diluent, and their numbers remained high after the sixth allergen challenge, although the difference was not significant (d). A significant inverse correlation was found between blood and skin tissue-infiltrating cDCs 24 h after the first allergen challenge (; ) (e). Data are expressed as median with interquartile range (first and third quartiles). *, WBC: whole blood cells.
