MicroRNA Dysregulation in Liver and Pancreas of CMP-Neu5Ac Hydroxylase Null Mice Disrupts Insulin/PI3K-AKT Signaling
Figure 5
Posttranscriptional regulation of identified target genes by dysregulated miRNAs (miR-15a or miR-28a and b). (a) HDAC4 transcript level as a positive or negative control was analyzed by RT-qPCR after transfection using 10 nM miR-1 mimic or AllStar negative control siRNA. Mock: no transfection of miRNA mimic, NC: negative control (transfection of 50 nM AllStar negative control siRNA), PC: positive control (transfection of 10 nM miR-1 mimic). (b) RT-qPCR analysis of miR-15a predicted target genes. The transfection of miR-15a-5p mimic into HepG2 cells downregulates putative target gene mRNAs expression. (c) and (d) RT-qPCR analysis of miR-29a or -29b predicted target genes. The transfections of miR-29a-3p mimic or miR-29b-3p mimic into HepG2 cells downregulate their putative target gene mRNAs expression, respectively. The experiments were independently repeated three times and GAPDH served as internal control. (; ; ).
