Extracts from Glioma Tissues following Cryoablation Have Proapoptosis, Antiproliferation, and Anti-Invasion Effects on Glioma Cells
Figure 2
Cryoablated tumor extracts induced GL261 glioma cell apoptosis via extrinsic pathway. (a) TUNEL assay detecting the apoptotic processes in the GL261 cells. GL261 cells were treated with cryoablated tumor extracts (1 mL extract solution/1 mL DMEM) for 12 h. Scale bar, 50 m. (b) Comparison of the number of apoptotic cells with the TUNEL assay. (c) Annexin V/PI staining and flow cytometry analysis assessing apoptosis in GL261 cells. (d) The levels of PARP, active caspase-8, procaspase-8, and procaspase-9 in GL261 cells analyzed via Western blot. (e) Apoptosis analysis in GL261 glioma cells treated with cryoablated tumor extracts after caspase-8, caspase-9, or P2X7 was blocked. GL261 cells were treated with cryoablated tumor extracts (1 mL extract solution/mL DMEM), together with the inhibitors of caspase-8 (10 M IETD), caspase-9 (10 M LEDH), and P2X7 (10 M A740003), respectively, for 12 h. Compared with the control group, .
