Adhesion of Pancreatic Cancer Cells in a Liver-Microvasculature Mimicking Coculture Correlates with Their Propensity to Form Liver-Specific Metastasis In Vivo
Figure 3
Images of the cells in the collagen gel cultures on day 4. (a) Monolayer morphology of 10T1/2 cells (red) without TMNK-1. (b) 10T1/2 cells (red) show a dispersed morphology containing cytoplasmic processes (indicated by arrows) with TMNK-1. (c) TMNK-1 cells (green) form monolayer over 10T1/2. (d) Haematoxylin and eosin (HE) staining of vertical cross section of TMNK-1 only culture. (e) HE staining of TMNK-1 + 10T1/2 culture. Some 10T1/2 cells in the culture can be distinguished by their elongated morphology beneath the TMNK-1 layer. Scale bars represent 200 μm (for (a), (b), and (c)) and 100 μm (for (d) and (e)).
