Construction of a Novel Liver-Targeting Fusion Interferon by Incorporation of a Plasmodium Region I-Plus Peptide

Figure 1

Schematic diagram of the gene synthesis method. The target DNA is dissected into oligos of between 29 and 58 nt long. Each four adjacent primers were mixed in a separate tube; after the first PCR, fragments overlap adjacent ones by up to 175 bp. In the overlap extension PCR step, the terminal fragments can be easily extended to full length.