Construction of a Novel Liver-Targeting Fusion Interferon by Incorporation of a Plasmodium Region I-Plus Peptide
Figure 3
Construction of fusion gene IFN-CSP and screening of recombinant plasmids containing fusion gene. (a) Fusion gene IFN-CSP was constructed by SOE-PCR. Lane M: DNA molecular weight marker. Lane 1–4: first PCR was performed for fusion gene assembly using eight pairs of oligonucleotides with different concentrations as templates to give PCR products ranging 132–175 bp in size. Lane 5: target amplification fragment of fusion gene IFN-CSP. (b) Screening of recombinant plasmids containing IFN-CSP gene by colony PCR and restriction endonuclease analysis. Lane M: DNA molecular weight marker, Lane 1: PCR products of recombinant plasmids, and Lane 2: Recombinant plasmids digested with Nde I/Xho I.
