Lipopolysaccharide Stimulates p62-Dependent Autophagy-Like Aggregate Clearance in Hepatocytes
Figure 2
LPS-mediated autophagy is independent of beclin1. (a) Immunoblot for beclin1 and LC3II expression in whole cell lysates from WT hepatocytes without treatment (0 h) or treated with LPS (100 ng/mL) for up to 24 h. (b) Immunoblot for beclin1 and LC3II in whole cell lysates from WT hepatocytes transfected with nontargeting, scrambled (control) siRNA, or siRNA targeting beclin1 and then treated with LPS (100 ng/mL) for up to 24 h. (c) Confocal immunofluorescence of GFP-LC3 in WT mouse hepatocytes transfected with GFP-LC3 and either control or beclin1 siRNA and treated with LPS (100 ng/mL) for 16 h. Data represents mean ± SEM; versus siRNA control (time 0) level. Images representative of at least 3 separate experiments. 60x magnification with 1.5x zoom. LC3 puncta was quantified and normalized by number of nuclei for ≥100 cells.
