Potential Role of A2B Adenosine Receptors on Proliferation/Migration of Fetal Endothelium Derived from Preeclamptic Pregnancies
Figure 6
Effect of stimulation in the VEGF protein level. (a) Representative images of western blot for VEGF (55 kDa) and β-actin (43 kDa) in HUVEC from normal or preeclamptic pregnancies incubated in absence (−) or presence of NECA (10 μM) and/or MRS-1754 (5 nM) during 12 hours. (b) Densitometry of the VEGF/β-actin ratio as showed in (a). (c) Representative images of VEGF (55 kDa) and β-actin (43 kDa) in HUVEC from normal pregnancies treated with NECA (10 μM) and/or MRS-1754 (5 nM) and/or L-NAME (100 μM) during 12 hours. (d) Densitometry of the VEGF/β-actin ratio as showed in (c). versus basal condition in normal pregnancy. Values are mean ± SEM. Values in respective control column indicate . All experiments were performed in duplicate.
