Analysis of Antigens of Mycobacterium leprae by Interaction to Sera IgG, IgM, and IgA Response to Improve Diagnosis of Leprosy
Figure 1
Bacterial index (BI) and MLF positive serum samples were used for calculating the best dilution of serum to recognize samples having anti-M. leprae antibodies. Results indicated that M. leprae infected human serum was reacted with the whole M. leprae sonicated antigen proteins where detector antibody was (a) IgG, (b) IgA, or (c) IgM. Examination of sera gives a general pattern found as above. Thus selected dilution of serum was 1 : 400 1 : 800 and 1 : 1600 for immunoblots with IgG, IgM, and IgA, respectively.
