Neuroprotective Effects of Geniposide in SH-SY5Y Cells and Primary Hippocampal Neurons Exposed to Aβ42

Figure 2

Effects of TLJN and its ingredients on neurite outgrowth of rat hippocampal neurons injured by Aβ. Rat hippocampal neurons were transfected with GFP for 3 days and observed under a fluorescence microscope (a) and its analog pictures were output (b, c). Bar = 25 μm (a, b). Neurites of the GFP-positive neurons cultured with the mixture of TLJN (high dose of TLJN: 5 μL TLJN per mL culture media (containing 83.5 μM geniposide and 8.35 μM ginsenoside Rg1); low dose of TLJN: 2 μL TLJN per mL culture media (containing 25.4 μM geniposide and 2.54 μM ginsenoside Rg1)) or its ingredient (geniposide (83.5 μM) and ginsenoside Rg1 (8.35 μM)) and Aβ (10 μM) were captured and drawn by using Image-Pro-Plus 5.0 software. Quantification of the length and number of neuritis of the neurons (d, e). Data were represented as mean standard deviation (SD); ^*, ^**, and ^***.