Necroptosis Mediates TNF-Induced Toxicity of Hippocampal Neurons
Figure 1
The regulation of RIP3 in TNF-α-induced toxicity of hippocampal neurons in vivo. (a) Nissl staining of hippocampal neurons 72 h after treatment. Wild-type (WT) and RIP3 knockout (KO) mice received intracerebroventricular injection of PBS or the indicated dose of TNF-α. The neurons of brain sections from WT and KO mice were analyzed by Nissl staining () and morphology of hippocampal CA3 region was shown. Arrows indicate the loss of hippocampal neurons. (b) and (c) Expressions of RIP1, RIP3, and caspase-3 in the hippocampus after TNF- α treatment. Proteins extracted from hippocampus in the wild-type mice treated with PBS or TNF-α were analyzed by western blot using indicated antibodies. PC: MEF cells were treated with staurosporine at 150 nM for 15 hours. The results shown here are representative of five mice.
