Necroptosis Mediates TNF-Induced Toxicity of Hippocampal Neurons

Figure 5

MLKL is essential for TNF-α-induced necrosis of HT-22 cells. (a) HT-22 cells were transfected with the negative control or MLKL siRNAs. After 60 h, cells were treated with control or TNF-α/z-VAD for another 20 h and then cell viability was determined by measuring ATP levels. Data were represented as mean ± standard deviation of duplicates. ^∗𝑃<0.01, ^∗∗𝑃<0.001 versus NC-T+Z. (b) The knockdown efficiency of MLKL RNAi. Cell lysates were collected 60 h after transfection and subjected to western blot analysis of MLKL and β-actin levels. All experiments were repeated three times with similar results.

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