Necroptosis Mediates TNF-Induced Toxicity of Hippocampal Neurons

Figure 6

TNF-α-induced necrosis of HT-22 cells is largely independent of ROS accumulation and calcium. (a) HT-22 cells were treated with control or BHA or NAC at the indicated concentration 3 h before the treatment of control or TNF-α/z-VAD for 20 h; cell viability was determined by measuring ATP levels. ^∗𝑃<0.01, ^∗∗𝑃<0.001 versus control-T+Z. (b) MEF cells were treated with control or BMS NAC at the indicated concentration 3 h before the treatment of control or TNF-α/Smac mimetic/z-VAD for 20 h; cell viability was determined by measuring ATP levels. ^∗𝑃<0.01, ^∗∗𝑃<0.001 versus control-T+S+Z. (c) HT-22 cells were treated with control or LaCl₃ or NAC at the indicated concentration 3 h before the treatment of control or TNF-α/z-VAD for 20 h; cell viability was determined by measuring ATP levels. Data are represented as mean ± standard deviation of duplicates. ^∗𝑃<0.01, ^∗∗𝑃<0.001 versus control-T+Z. All experiments were repeated three times with similar results.

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