Functional Screening of Antibiotic Resistance Genes from a Representative Metagenomic Library of Food Fermenting Microbiota
Figure 2
Fosmid cloning of total MBC DNA does not alter complexity PCR amplification of total DNA extracted from pooled MBC samples (a) or from pooled recombinant fosmids following metagenomic library construction (b). Primer pairs: bacterial rDNA, yeast rDNA, tet(M), and tet(S) (Table 1). M: 1 Kb DNA ladder.