Research Article
Functional Screening of Antibiotic Resistance Genes from a Representative Metagenomic Library of Food Fermenting Microbiota
Table 2
Summary of screening procedure and resulting AR recombinant library clones.
| Pharmacological class | Antibiotic | Target | E. coli MIC (mg/L) | Library clones identified | Verified by secondary screening |
| Tetracyclines | Tetracycline | Ribosome | 5 | 4 | 0 | Aminoglycosides | Kanamycin , gentamycin | Ribosome | 25 12 | 2 0 | 2 — | Macrolides | Erythromycin | Ribosome | Resistant | — | — | Beta-lactams | Ampicillin | Cell wall | 25 | 6 | 6 | Glycopeptides | Vancomycin | Cell wall | Resistant | — | — |
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