Research Article
Functional Screening of Antibiotic Resistance Genes from a Representative Metagenomic Library of Food Fermenting Microbiota
Table 2
Summary of screening procedure and resulting AR recombinant library clones.
| | Pharmacological class | Antibiotic | Target | E. coli MIC (mg/L) | Library clones identified | Verified by secondary screening |
| | Tetracyclines | Tetracycline | Ribosome | 5 | 4 | 0 | | Aminoglycosides | Kanamycin ,
gentamycin | Ribosome | 25
12 | 2
0 | 2
— | | Macrolides | Erythromycin | Ribosome | Resistant | — | — | | Beta-lactams | Ampicillin | Cell wall | 25 | 6 | 6 | | Glycopeptides | Vancomycin | Cell wall | Resistant | — | — |
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