Functional Screening of Antibiotic Resistance Genes from a Representative Metagenomic Library of Food Fermenting Microbiota

Table 3

Summary of insert sequencing results of AR fosmids. ORFs with a possible function in AR, as well as transposase genes, are listed.

	Amp1	Amp3	Amp6	Kan10
Insert length (bp)	14.380	38.386	21.644	33.491
Predicted ORFs ()	14	43	21	35
Species	S. thermophilus	S. thermophilus	S. thermophilus	S. thermophilus
Relevant ORFs for AR	Serine endopeptidase	Penicillin-binding protein 2A; Penicillin-binding protein 1A; RNA methyltransferase; GNAT family acetyltransferase	Phosphoglucomutase	23S rRNA methyltransferase
Transposase sequences ()	2	5	10	1
MIC of the corresponding antibiotic (mg/L)	50	50	50	25