Trehalose-Based Eye Drops Preserve Viability and Functionality of Cultured Human Corneal Epithelial Cells during Desiccation

Table 2

Results from live/dead assay at all time points, shown as mean percentage of living cells, with standard deviation (st. dev.), max and min, and P value in comparison to 0.9% NaCl control.

(a)

	5 min					15 min
	Mean [%]	St. dev.	Max	Min	P value	Mean [%]	St. dev.	Max	Min	P value
Thealoz	98.77	0.45	99.2	98.3	<0.01	90.65	2.05	92.1	89.2	<0.01
Hialeye 0.2	92.63	0.75	93.4	91.9	<0.05	35.1	2.26	36.7	33.5	<0.01
Hialeye 0.4	87.37	2.34	89.1	84.7	ns	32.1	0.28	32.3	31.9	<0.01
Keratostill	92	1.1	93.1	90.9	ns	50.05	3.04	52.2	47.9	<0.01
Lacrimal	40.1	1.34	41.2	38.6	<0.01	10.25	1.48	11.3	9.2	<0.01
Starazolin	91.53	1.62	93.3	90.1	ns	44.05	0.35	44.3	43.8	<0.01
Systane	92.9	0.36	93.2	92.5	<0.05	50	0.28	50.2	49.8	<0.01
0.9% NaCl	88.43	1.17	89.7	87.4		2.4	1.13	3.2	1.6
Not treated	89.63	1.12	90.6	88.4		0	0	0	0

(b)

	30 min					45 min
	Mean [%]	St. dev.	Max	Min	P value	Mean [%]	St. dev.	Max	Min	P value
Thealoz	51.75	1.48	52.8	50.7	<0.01	10.25	2.76	12.2	8.3	<0.01
Hialeye 0.2	8.15	0.35	8.4	7.9	<0.01	0.6	0.85	1.2	0	ns
Hialeye 0.4	11.2	1.55	12.3	10.1	<0.01	0	0	0	0	ns
Keratostill	23.5	2.83	25.5	21.5	<0.01	4.45	1.77	5.7	3.2	<0.01
Lacrimal	0	0	0	0	ns	0	0	0	0	ns
Starazolin	7.85	0.35	8.1	7.6	<0.01	0	0	0	0	ns
Systane	6.85	3.32	9.2	4.5	<0.01	0.6	0.85	1.2	0	ns
0.9% NaCl	0	0	0	0		0	0	0	0
Not treated	0	0	0	0		0	0	0	0