Preparation of Three-Dimensional Vascularized MSC Cell Sheet Constructs for Tissue Regeneration

Figure 1

The schematic describes the procedures of preparing a three-dimensional prevascularized construct based on a modified cell sheet engineering technique. A cell scraper was used to scratch the cell sheet and a point forceps was then used to fold the cell sheet 3 times to form a square-like construct with 8 layers. The width was approximately 8 mm and length was around 8 mm.