Bioactivity-Guided Fractionation Identifies Amygdalin as a Potent Neurotrophic Agent from Herbal Medicine Semen Persicae Extract
Figure 5
Activation of ERK1/2 pathway by amygdalin and amygdalin-containing fractions. (a) Effect of amygdalin on the phosphorylation of ERK1/2. PC12 were treated with 20 μM of amygdalin for indicated times or different concentrations of amygdalin (0–20 μM) for 1 hour. Total and phosphorylated ERK1/2 were detected by Western blotting analysis. (b) Effect of amygdalin-containing fractions on the phosphorylation of ERK1/2. For upper panel, PC12 were treated with Semen Persicae extract (1 mg/mL) for indicated times. For lower panel, PC12 were treated with amygdalin (20 μM) or F5 (1 mg/mL relative to the parent Semen Persicae extract) for 60 minutes. Total and phosphorylated ERK1/2 were detected by Western blotting analysis. Three independent experiments were performed. The mean values for fold of induction were shown under each treatment.
