Research Article
Development and Characterization of New Monoclonal Antibodies against Human Recombinant CA XII
Table 2
Characterization of the MAbs raised against recombinant CA XII: summarized data.
| | | | | | Reactivity with CA XII: | | MAb clone |
Cross-reactivity of the MAbs with recombinant CA isoforms | IgG subtype | Apparent , |
expressed in E. coli |
expressed in mammalian cells |
in cancer cells or tissue | | | CA I | CA II | CA VII | CA XIII | | | ELISA | WB | ELISA | WB | FACS | IHC |
| | 1D5 | − | − | − | − | IgG1 | 2.07 × 10−10 | + | + | − | − | − | − | | 4A6 | − | − | − | − | IgG1 | 1.07 × 10−9 | + | + | + | + | − | −/+ | | 5D2 | − | − | − | − | IgG1 | 5.45 × 10−10 | + | + | − | − | − | − | | 8C9 | − | − | − | − | IgG1 | 1.88 × 10−10 | + | + | −/+ | + | − | − | | 9A8 | − | − | − | − | IgG1 | 6.17 × 10−10 | + | + | + | + | − | − | | 13F5 | − | −/+ | −/+ | − | IgG1 | 1.12 × 10−10 | + | + | −/+ | + | − | − | | 15A4 | − | − | − | − | IgG2a | 2.02 × 10−10 | + | + | + | + | + | + |
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“+”: strong reaction, “−/+”: weak reaction, “−”: no reaction.
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