Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development
Figure 2
Fin bud and tail defects caused by injection of pax1b MO and/or zebrafish pax9 MO. (a) Fin bud and axis defects caused by injection of 5 ng pax1b MO. (b) Tail defect caused by injection of 5 ng zebrafish pax9 MO. (c) Fin bud and tail defects caused by coinjection of 2.5 ng pax1b MO and 2.5 ng zebrafish pax9 MO. (d) Statistics of phenotype ratios. White columns indicate the ratio of fin bud defect; shaded columns indicate the ratio of kinky tail. (e) Statistical analysis of phenotypes caused by coinjection of different mRNAs with 5 ng zebrafish pax9 MO. The amount of mRNA injected for every embryo is as follows: 200 ng gfp mRNA, 200 ng pax1b mRNA, 200 ng pax1a mRNA, and 200 ng zebrafish pax9 mRNA. All embryos were observed at 36 hpf.
