Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development
Figure 6
The interaction between PAX1 and FOXO1. (a) FOXO1 is a new PAX1-interacting protein. HEK293FT cells were transfected with Myc-tagged FOXO1 and Flag-tagged PAX1 cloned from human cDNA. Cells were harvested for immunoprecipitation with anti-Flag affinity resin and immunoblotted with the indicated antibodies. (b) The interaction of Pax1 and FOXO1 was conserved. Myc-tagged FOXO1 was immunoprecipitated by Flag-tagged Pax1b or mouse Pax1. (c) PAX9 did not interact with FOXO1 in HEK293FT cells. zPax1b: zebrafish Pax1b; mPax1: mouse Pax1. TCL: total cell lysate. IP: immunoprecipitation.
