Characterization of Zebrafish Pax1b and Pax9 in Fin Bud Development
Figure 7
The interaction between PAX1 and FOXO1 is enhanced under hypoxia stress. (a) Colocalization of PAX1 and FOXO1 in HeLa cells. Myc-FOXO1 is distributed in the cytoplasm and nucleus while Flag-PAX1 is located in the nucleus only. The colocalization of PAX1 and FOXO1 was increased after treatment with 20 μM CoCl2 for 14 h. Myc-FOXO1 was cotransfected with Flag-PAX1 into HeLa cells. 36 h after transfection, cells were subjected to immunostaining using anti-Myc antibody, anti-Flag antibody, and DAPI and observed by microscopy. ((b)-(c)) 2 μg Flag-tagged PAX1 and/or 0.5 μg Myc-tagged FOXO1 were transfected into HEK293FT cells, respectively. Semiendogenous Co-IP revealed that the interaction of PAX1 and FOXO1 was strengthened when stimulated with CoCl2 and the enhancement occurred in a dose-dependent manner.
