Cysteine Cathepsin Activity Regulation by Glycosaminoglycans

Figure 3

Predicted GAG-binding sites in papain-like peptidases. (a) Three predicted CS-binding sites in cathepsin S. (b) Two predicted HS/HP-binding sites in cathepsin B. (c) The conserved GAG-binding motif in papain. Predicted sites are shown in circles and positively charged residues at each site are shown as blue sticks and labeled. The position of the active site cleft is marked by an arrow. All coordinates were obtained from the Protein Data Bank (accession codes: 1NQC for cathepsin S, 3AI8 for cathepsin B, and 1PPN for papain, resp.). All images were created with PyMOL.

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