Infliximab Reverses Suppression of Cholesterol Efflux Proteins by TNF-α: A Possible Mechanism for Modulation of Atherogenesis
Figure 1
ABCA1 message (a) and protein (c) levels in THP-1 monocytes decrease with exposure to TNF- and this effect is reversed by infliximab. mRNA level (b) and protein expression (d) of ABCG1 did not change in the presence of TNF- or infliximab. THP-1 monocytes were incubated under the following 5 conditions: (1) RPMI alone (Control), (2) IFN- (500 U/mL), (3) TNF- (100 U/mL), (4) TNF- (100 U/mL) + infliximab (5 g/mL), and (5) infliximab (5 g/mL). Quantitative analysis for changes in ABCA1 and ABCG1 mRNA level was performed using RT-PCR with GAPDH message as an internal standard. Protein expression was analyzed by Western blot and band intensities were calculated using β-actin as a loading control. All results are presented as means ± SEM of three independent experiments. *; **; *** versus control; #; ### versus TNF-α treated cells.
