Infliximab Reverses Suppression of Cholesterol Efflux Proteins by TNF-α: A Possible Mechanism for Modulation of Atherogenesis
Figure 2
The effect of TNF- and infliximab on 27-hydroxylase (27-OH) message (a) and protein (b) levels in THP-1 monocytes. THP-1 monocytes were incubated under the following 5 conditions: (1) RPMI alone (Control), (2) IFN- (500 U/mL), (3) TNF- (100 U/mL), (4) TNF- (100 U/mL) + infliximab (5 g/mL), and (5) infliximab (5 g/mL). Quantitative analysis for changes in ABCA1 mRNA level was performed using RT-PCR with GAPDH message as an internal standard. Protein expression analysis was performed using Western blot and band intensities were calculated using β-actin as a loading control. All results are presented as means ± SEM of three independent experiments. **; versus control cells.
