The β-SiC Nanowires (~100 nm) Induce Apoptosis via Oxidative Stress in Mouse Osteoblastic Cell Line MC3T3-E1
Figure 2
The effect of β-SiC nanowires on the cytotoxicity of MC3T3-E1 cells. (a) After 24 h exposure to 6.25, 12.5, 25, 50, and 100 μg/mL of β-SiC nanowires (100 nm, 100 μm), MTT assay was used to evaluate the viability of MC3T3-E1 cells. (b) Representative photographs of double staining of PI and Hoechst 33342. The apoptotic cells were observed as PI intense signal after double staining. (c) Cells were stained with annexin V-FITC and PI, analyzed by flow cytometry. H2O2 treated cells were used as positive control. (d) Cells were treated with 12.5 μg/mL of 100 nm long β-SiC nanowires (100 nm) for 12 h, 24 h, and 48 h. The distribution of viable, early apoptotic, later apoptotic, and necrotic cells was analyzed. The data are expressed as percentage of total cells. (e) The expression of apoptotic genes was analyzed by western blot in MC3T3-E1 cells. Values are the means ± SD () of three individual experiments. (f) Gray value percentage of target protein and β-actin. , versus ctr (0 μg/mL).
