The β-SiC Nanowires (~100 nm) Induce Apoptosis via Oxidative Stress in Mouse Osteoblastic Cell Line MC3T3-E1

Figure 4

Effects of different β-SiC nanowires on energy metabolism in MC3T3-E1 cells. TEM image of β-SiC nanowires internalization in MC3T3-E1 cells treated with 12.5 μg/mL of 100 nm long β-SiC nanowires ((a)–(d)). Scale bar = 2 μm; red arrows indicate nanowires internalization. Before the oxygen consumption test (e), MC3T3-E1 cells were pretreated in low oxygen conditions for 2 h in suspension culture with different β-SiC nanowires. (f) The intracellular ATP concentrations were evaluated after exposure to different β-SiC nanowires for 24 h. Values are the means ± SD (𝑛=3) of three independent experiments. ^∗𝑃<0.05, ^∗∗𝑃<0.01 versus control.