Antioxidant/Prooxidant and Antibacterial/Probacterial Effects of a Grape Seed Extract in Complex with Lipoxygenase

Figure 2

Cellular respiration (as measured by the mitochondrial MTT conversion to formazan) after coincubation with different mixtures of polyphenols (CS and PE) and lipoxygenases (LS and LE) for different periods (3 hs and 24 hs). Treatment with the pure catechin (CS to a final concentration of 310 µM/mL medium) and grape seed extract (PE to a final concentration of 35 µg polyphenols/mL medium) was administrated at the initiation of the culture (3 hrs and 24 hrs for 3 hrs) and at 24 h (24 hrs). Leucocyte respiration was assessed 4 h (3 hrs) and 24 h (24 hrs for 3 hrs) after adding the polyphenols (CS and PE) for the same cell sample and at 25 h (24 hrs) for another one. Data are presented as mean ± S.E.M. Values are expressed as percent of control for the experimental variants tested: LS-standard lipoxygenase, LE-lipoxygenase extract, CS-catechin standard, and PE-polyphenolic extract.