Synergistic Effects of Orbital Shear Stress on In Vitro Growth and Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells

Figure 10

Representative optical fluorescence microscopy images of hABMSCs cultured for 5 days in static conditions (a1–d1) or at 10 min/day (a2–d2), 30 min/day (a3–d3), 60 min/day (a4–d4), 120 min/day (a5–d5), and 180 min/day (a6–d6) by OSS with OM; cell nuclei (a1–a6), actin filaments (b1–b6), gap junction (Cx43, c1–c6), and merged images (d1–d6) of the fluorescence stains. Fluorescence images showed more intense observation at OSS groups compared to those of control (arrows: cell direction).